In order to explore the reasonable initial tension of the cable-stayed cable of the mixed-structure cable-stayed bridge with special-shaped tower, and further optimize the distribution of the internal force of the cable-stayed cable in the state of the bridge, a span of special-shaped tower mixed structure cable-stayed bridge is arranged as (2×30) + (2×60) + (2×30)m based on the principle of influence matrix, the initial tension of the cable-stayed cable is optimized and analyzed, the reasonable initial tension of the cable-stayed cable is calculated; while taking the designed bridge cable force and the optimized initial tension as the initial tension of the cable-stayed cable, the response of the bridge structure under different initial tension is compared and analyzed. The results show that: the influence matrix method has a good effect on the optimization of the initial tension of the cable-stayed bridge of the mixed structure of the special-shaped tower. The internal force of the optimized cable-stayed cable tends to be the design bridge force, and after the initial tensile force is optimized, the overall distribution of the bending moment of the main girder of the cable-stayed bridge with mixed structure of special-shaped tower is more uniform, and the overall vertical displacement of the main girder is reduced by 38.2% compared with that before optimization, which not only improves the structural force, but also plays a controlling role in the bridge alignment. The results can provide a certain reference for optimization of the initial tension of cable-stayed cables for the same type of bridges.