During the construction period of Yunnan road station on nanjing metro line 5, the protection of large-sized 110 kV cable pipe culverts is of vital importance. After a comprehensive comparison of the construction period, cost and risk, the article decided to optimize the structural dimensions and construction sequence of the station and carry out overall in-situ protection for the 110kV cable pipe culvert. The protection design scheme adopted the structure of steel beams and columns (partially utilizing the existing retaining piles of the station), and calculated based on the load and force system. Determine the size of the steel section piles. And the connection mode of beam-column joints is refined; During the on-site construction period, the depth of the steel section piles is optimized through in-situ tests. Arc-shaped steel plates are pre-embedded in the retaining piles to ensure effective connection with the steel section corbels. Measures such as padding steel wedges and filling with fine aggregate concrete are adopted to effectively reduce the deformation of cables, pipes and culverts. Refined management is carried out and on-site inspection and monitoring are strengthened. The results show that under the controllable conditions of engineering risks and environmental risks, the cost and construction period of the overall in-situ protection of pipelines have advantages. The protection plan should be comprehensively considered in combination with the station design plan and the construction sequence. During the implementation process, the plan can be further optimized through tests.